Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Efficient Production of Broad-Spectrum Antimicrobials by Paenibacillus polymyxa OSY–EC Using Acid Whey-Based Medium and Novel Antimicrobial Concentration Approach

doi: 10.3389/fbioe.2022.869778

Figure Lengend Snippet: Release of the intracellular potassium ions from Listeria innocua ATCC 33090 (panel A ) and Escherichia coli k12 (panel B ) treated with solutions containing different concentrations (0–1,600 μg/ml) of antimicrobials-rich powder (OSY–EC AMRP), prepared from acid whey-based medium inoculated with Paenibacillus polymyxa OSY–EC. The release of intracellular K + was measured as changes in fluorescence at excitation/emission wavelength of 346/505 nm. Data are represented as mean ± standard deviation.

Article Snippet: Eluted peptides were detected using a multi-UV detector at a 215 nm wavelength (NGCTM Multi-Wavelength Detector; Bio-Rad).

Techniques: Fluorescence, Standard Deviation

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Efficient Production of Broad-Spectrum Antimicrobials by Paenibacillus polymyxa OSY–EC Using Acid Whey-Based Medium and Novel Antimicrobial Concentration Approach

doi: 10.3389/fbioe.2022.869778

Figure Lengend Snippet: The uptake of 1- N -phenylnaphthylamine (NPN) by Escherichia coli k12 treated with solutions containing different concentrations (0–1,600 μg/ml) of antimicrobials-rich powder (OSY–EC AMRP), prepared from acid whey-based medium inoculated with Paenibacillus polymyxa OSY–EC. The outer membrane disruption in E. coli k12 by the peptides was measured as changes in fluorescence at excitation/emission wavelength of 340/405 nm. Data are represented as mean ± standard deviation.

Article Snippet: Eluted peptides were detected using a multi-UV detector at a 215 nm wavelength (NGCTM Multi-Wavelength Detector; Bio-Rad).

Techniques: Membrane, Disruption, Fluorescence, Standard Deviation

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Efficient Production of Broad-Spectrum Antimicrobials by Paenibacillus polymyxa OSY–EC Using Acid Whey-Based Medium and Novel Antimicrobial Concentration Approach

doi: 10.3389/fbioe.2022.869778

Figure Lengend Snippet: The uptake of Sytox™ green dye by Listeria innocua ATCC 33090 (panel A ) and Escherichia coli k12 (panel B ) treated with solutions containing different concentrations (0–1,600 μg/ml) of antimicrobials-rich powder (OSY–EC AMRP), prepared from acid whey-based medium inoculated with Paenibacillus polymyxa OSY–EC. The inner membrane disruption by the peptides was measured as changes in fluorescence at excitation/emission wavelength of 488/530 nm. Data are represented as mean ± standard deviation.

Article Snippet: Eluted peptides were detected using a multi-UV detector at a 215 nm wavelength (NGCTM Multi-Wavelength Detector; Bio-Rad).

Techniques: Membrane, Disruption, Fluorescence, Standard Deviation